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Human cell-surface glycoprotein with unusual properties

M B Omary, I S Trowbridge, J Minowada

    Nature
    |August 28, 1980
    PubMed
    Summary

    Researchers identified a human cell-surface glycoprotein present on cultured hematopoietic cells but not most normal cells. Its expression decreases upon HL-60 cell differentiation, suggesting a link to cell proliferation and malignancy.

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    Area of Science:

    • Cell Biology
    • Immunology
    • Oncology

    Background:

    • A novel human cell-surface glycoprotein was detected on cultured hematopoietic cell lines.
    • This glycoprotein is expressed on all tested cultured hematopoietic cell lines, including HL-60.
    • It is notably absent or present in low amounts on most normal or leukemic hematopoietic cells.

    Purpose of the Study:

    • To identify and characterize a novel human cell-surface glycoprotein.
    • To investigate the expression patterns of this glycoprotein in various cell types and conditions.
    • To explore the potential role of this glycoprotein in cell differentiation and malignancy.

    Main Methods:

    • Monoclonal antibody-based detection of cell-surface glycoproteins.
    • Analysis of glycoprotein expression on cultured human hematopoietic cell lines (e.g., HL-60).
    • In vitro induction of HL-60 cell differentiation using chemical inducers.
    • Expression analysis on tumor cell lines from non-hematopoietic tissues.
    • Preliminary chemical characterization of the identified glycoprotein.

    Main Results:

    • The glycoprotein is present on all cultured human hematopoietic cell lines tested.
    • HL-60 cells lose this glycoprotein upon in vitro differentiation into granulocytes or macrophages.
    • The glycoprotein is also found on tumor cell lines from non-hematopoietic tissues, indicating it is not strictly tissue-specific.
    • Its expression appears to correlate with cell proliferation rather than normal differentiation.
    • Preliminary data suggests it may be identical to a previously reported malignancy-associated glycoprotein.

    Conclusions:

    • The identified glycoprotein is a cell-surface marker with unusual expression patterns.
    • Its expression is linked to cell proliferation and potentially malignancy, rather than normal hematopoietic differentiation.
    • Further investigation is warranted to confirm its identity and role in cancer.

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