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Related Experiment Videos

Increased creatine kinase activities associated with haemolysis

R Bais, J B Edwards

    Pathology
    |April 1, 1980
    PubMed
    Summary

    Haemolysis falsely elevates creatine kinase activity due to adenylate kinase from red blood cells. Adding diadenosine pentaphosphate corrects this interference, ensuring accurate enzyme measurements.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Enzymology

    Background:

    • Creatine kinase (CK) is a crucial enzyme marker in clinical diagnostics.
    • Haemolysis, the rupture of red blood cells, can interfere with laboratory assays.
    • Adenylate kinase (AK) released during haemolysis is known to affect CK measurements.

    Purpose of the Study:

    • To investigate the impact of haemolysis on creatine kinase activity.
    • To identify the specific interfering substance released from erythrocytes.
    • To find a method to correct for this interference in CK assays.

    Main Methods:

    • Investigating the effect of haemolysis on creatine kinase activity in vitro.
    • Analyzing the role of adenylate kinase in apparent creatine kinase elevation.
    • Testing the efficacy of diadenosine pentaphosphate in mitigating the interference.

    Main Results:

    • Haemolysis was found to increase apparent creatine kinase activity.
    • Adenylate kinase released from erythrocytes was identified as the cause of this increase.
    • Addition of 10 mumol/l diadenosine pentaphosphate effectively eliminated the interference.

    Conclusions:

    • Haemolysis significantly impacts creatine kinase assay accuracy.
    • Adenylate kinase interference can lead to falsely elevated CK levels.
    • Diadenosine pentaphosphate is a reliable inhibitor for correcting AK interference in CK assays.

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