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Stability of human plasma catecholamines

J Pettersson, E Hussi, J Jänne

    Scandinavian Journal of Clinical and Laboratory Investigation
    |June 1, 1980
    PubMed
    Summary
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    Human plasma catecholamines (adrenaline and noradrenaline) are stable when stored in whole blood at room temperature. Antioxidants are not required, simplifying sample handling for accurate measurements.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Pharmacology

    Background:

    • Plasma catecholamines are crucial biomarkers.
    • Previous studies suggested instability and required antioxidants for storage.
    • Accurate measurement necessitates stable sample integrity.

    Purpose of the Study:

    • To evaluate the stability of human plasma catecholamines (adrenaline and noradrenaline) during storage.
    • To determine the necessity of antioxidants and specific storage conditions.
    • To compare findings with previous reports on catecholamine stability.

    Main Methods:

    • Radioenzymatic assay for measuring plasma catecholamines.
    • Storage of whole blood and plasma samples at room temperature.
    • Assessment of catecholamine levels after varying storage durations.

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  • Inclusion of antioxidant-free and antioxidant-supplemented conditions.

    • Main Results:

    • Plasma catecholamines (adrenaline, noradrenaline) demonstrated marked stability in whole blood stored at room temperature for several hours.
    • No loss of catecholamines was observed without prior plasma separation.
    • Antioxidants like thiol compounds or sodium metabisulphite were not required for storage stability.
    • Catecholamines degraded rapidly in buffer solutions without thiols.
    • An anomalous, unexplained increase in adrenaline and noradrenaline was noted in one subject within 30 minutes of storage.

    Conclusions:

    • Human plasma catecholamines exhibit significant stability in whole blood at room temperature, challenging prior assumptions.
    • The routine use of antioxidants and stringent sample processing may be re-evaluated based on observed stability.
    • Further investigation is warranted for the anomalous increase in catecholamine concentrations observed in specific cases.