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N-Nitrosodiethanolamine revisited

J R Andersen, W Batsberg, L Carlsen

    Biomedical Mass Spectrometry
    |May 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

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    N-nitrosodiethanolamine (NDELA) is a suspected carcinogen found in consumer products. This study presents a reliable purification method using high-performance liquid chromatography (HPLC) to ensure accurate chemical analysis and safety assessments.

    Area of Science:

    • Analytical Chemistry
    • Chemical Toxicology
    • Spectroscopy

    Background:

    • N-nitrosodiethanolamine (NDELA) is a widely occurring chemical suspected of weak carcinogenicity.
    • Concerns regarding human health hazards exist due to NDELA's presence in consumer products.
    • Incomplete chemical data on NDELA's identity, purity, and properties hinders accurate risk assessment.

    Purpose of the Study:

    • To develop and present an effective purification procedure for synthetic N-nitrosodiethanolamine.
    • To establish the purity and identity of purified NDELA using advanced analytical techniques.
    • To address the limitations of existing fractionation methods for NDELA analysis.

    Main Methods:

    • High-performance liquid chromatography (HPLC) was employed as the primary purification technique.

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  • Gas liquid chromatography, ambient pressure column chromatography, and distillation were evaluated and found inadequate.
  • Purity and identity were confirmed using electron impact and field ionization mass spectrometry (MS), including metastable defocusing and collision-induced decomposition.
  • Nuclear magnetic resonance (1H and 13C NMR), infrared (IR), and ultraviolet (UV) spectroscopy were utilized.
  • Deuterium-labeled analogues of NDELA and diethanolamine aided in result interpretation.

    • Main Results:

    • A robust purification method for synthetic N-nitrosodiethanolamine based on HPLC was successfully developed.
    • HPLC proved superior to other tested fractionation techniques for NDELA purification.
    • Comprehensive spectroscopic analyses confirmed the identity and high purity of the refined NDELA.

    Conclusions:

    • The developed HPLC method provides a reliable means to obtain pure N-nitrosodiethanolamine for accurate chemical and toxicological studies.
    • This purification advancement is crucial for resolving uncertainties surrounding NDELA's properties and its potential human health risks.
    • The findings support further investigation into NDELA's carcinogenicity with a well-characterized substance.