Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell substrate adhesiveness in S49 mouse lymphoma

J Hochman, A Katz, E Levy

    Cell Biology International Reports
    |October 1, 1980
    PubMed
    Summary

    A new S49-Adh mouse lymphoma cell line exhibits enhanced substrate adhesiveness, enabling monolayer growth. This trait remains stable through extended in vitro culture and in vivo passage, indicating a robust genetic characteristic.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A novel retro-inverso gonadotropin-releasing hormone (GnRH) immunogen elicits antibodies that neutralize the activity of native GnRH.

    Endocrinology·2003
    Same author

    Noninvasive native fluorescence imaging of head and neck tumors.

    Technology in cancer research & treatment·2003
    Same author

    Variation in the DCP1 gene, encoding the angiotensin converting enzyme ACE, is not associated with increased susceptibility to Alzheimer's disease.

    Psychiatric genetics·2003
    Same author

    Therapeutic potential of curcumin in human prostate cancer-I. curcumin induces apoptosis in both androgen-dependent and androgen-independent prostate cancer cells.

    Prostate cancer and prostatic diseases·2002
    Same author

    Interpretation of spring recession curves.

    Ground water·2002
    Same author

    Ideal vitamin C intake.

    BioFactors (Oxford, England)·2002

    Area of Science:

    • Cell Biology
    • Genetics
    • Cancer Research

    Background:

    • S49 mouse lymphoma cells are typically grown in suspension culture.
    • Cellular adhesion plays a crucial role in tissue formation and cellular behavior.
    • Understanding genetic traits influencing cell adhesion can provide insights into cancer progression.

    Purpose of the Study:

    • To characterize a novel subline of S49 mouse lymphoma cells with altered adhesive properties.
    • To investigate the stability of the substrate-adhesive phenotype in S49-Adh cells.
    • To explore the implications of enhanced cell adhesion in a lymphoma model.

    Main Methods:

    • Selection of a variant subline from S49 mouse lymphoma cells.
    • Cultivation of cells in suspension and as monolayers.
    • Assessment of cell-substrate adhesion.
    • In vitro cultivation and in vivo passage in nude mice for phenotype stability testing.

    Main Results:

    • A new subline, S49-Adh, was isolated, displaying increased substrate adhesiveness.
    • S49-Adh cells can grow as a monolayer, unlike parental suspension cells.
    • The substrate-adhesive phenotype of S49-Adh cells proved stable across extended in vitro and in vivo conditions.
    • Daughter cells from a dense monolayer retained substrate adhesiveness while also forming suspension populations.

    Conclusions:

    • Enhanced cell-substrate adhesiveness is a stable, genetically determined trait in the S49-Adh cell line.
    • This variant provides a model for studying the genetic basis and functional consequences of cell adhesion in lymphoma.
    • The ability to switch between monolayer and suspension growth while retaining adhesion warrants further investigation.

    Related Experiment Videos