Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Cofactor in serum for high-affinity folate binding in milk

J Holm, S I Hansen, J Lyngbye

Clinical Chemistry

|October 1, 1980

Summary

A heat-resistant cofactor crucial for high-affinity folate binding was identified in cow's milk whey. This cofactor, also found in human sera and red blood cells, modulates folate binding cooperativity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The continuous reaction time test for minimal hepatic encephalopathy validated by a randomized controlled multi-modal intervention-A pilot study.

PloS one·2017

Same author

The effect of amiodarone on warfarin anticoagulation: a register-based nationwide cohort study involving the Swedish population.

Journal of thrombosis and haemostasis : JTH·2017

Same author

Regional contribution to ventricular stroke volume is affected on the left side, but not on the right in patients with pulmonary hypertension.

The international journal of cardiovascular imaging·2016

Same author

Breast cancer genetic risk profile is differentially associated with interval and screen-detected breast cancers.

Annals of oncology : official journal of the European Society for Medical Oncology·2016

Same author

Comparative Investigation of Postoperative Complications in Patients With Gastroesophageal Junction Cancer Treated With Preoperative Chemotherapy or Surgery Alone.

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2015

Same author

Breast cancer genetic risk profile is differentially associated with interval and screen-detected breast cancers.

Annals of oncology : official journal of the European Society for Medical Oncology·2014

Area of Science:

Biochemistry
Molecular Biology
Nutritional Science

Background:

High-affinity folate binding is essential for cellular processes.
A heat-resistant cofactor influencing folate binding was previously observed in cow's milk whey.
The precise role and identity of this cofactor remain largely unknown.

Purpose of the Study:

To investigate the presence and activity of a heat-resistant cofactor in human biological samples.
To determine if this cofactor modulates folate binding affinity and cooperativity in humans.
To explore the potential physiological significance of this cofactor in folate metabolism.

Main Methods:

Affinity-chromatographic purification of folate-binding protein from cow's milk whey.
Assays to detect cofactor activity in human serum and erythrocyte hemolysates.
Analysis of folate binding cooperativity in the presence and absence of the cofactor.

Main Results:

Purification of folate-binding protein removed a heat-resistant cofactor essential for high-affinity binding.
The cofactor induced a shift from negative to positive cooperativity in folate binding.
A similar active cofactor was detected in human serum (men, pregnant women, umbilical cord) and diluted erythrocyte hemolysates.

Conclusions:

A heat-resistant cofactor modulating high-affinity folate binding is present in human biological fluids and cells.
This cofactor plays a significant role in the regulation of folate binding.
Further research is needed to elucidate the identity and precise function of this important cofactor.

Related Experiment Videos