Steady-state distribution of lithium during cultivation of dissociated brain cells

The formation of a steady-state intracellular lithium level was studied in the course of cultivation of dissociated nerve cell cultures obtained from chick embryonic brains. When lithium was given at a concentration of 2 mM, in the nutrient medium, at day 5, a steady state intracellular lithium content was achieved after about 30 min of incubation and it did not change significantly during the time of cultivation up to the 13th day in vitro.