Freezing and transplantation of brain tissue in rats

Neocortical tissue obtained from rat embryos was frozen and stored at - 70 degrees C for 6 h prior to transplantation into the cerebellum of neonatal rats. Growth, differentiation, and integration of this tissue within the host brain was comparable to that obtained from freshly dissected and transplanted tissue. It is suggested that freezing to low temperatures does not adversely effect the viability or transplantability of the neural tissue.