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Related Experiment Videos

Calcium binding to mastocyte plasma membrane

G Tolone, L Bonasera, F Vitale

    International Archives of Allergy and Applied Immunology
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

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    Rat mast cells have two main calcium binding sites on their plasma membrane. Phospholipids and neuraminic acid residues are key to this calcium binding.

    Area of Science:

    • Biochemistry
    • Cell Biology

    Background:

    • Rat mastocyte plasma membranes exhibit complex calcium interactions.
    • Understanding calcium binding is crucial for mast cell function.

    Purpose of the Study:

    • To characterize the calcium binding sites on rat mastocyte plasma membranes.
    • To quantify calcium binding affinity and capacity.

    Main Methods:

    • Utilized techniques to measure calcium binding to isolated rat mastocyte plasma membranes.
    • Analyzed binding data to determine affinity and capacity of different binding sites.

    Main Results:

    • Identified two major classes of calcium binding sites with distinct affinities.
    • Low-affinity sites bind 88 nmol Ca2+/mg protein (half-saturated at 125 µM Ca2+).

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  • High-affinity sites bind 12 nmol Ca2+/mg protein (half-saturated at 5.5 µM Ca2+).
  • Membrane phospholipids and neuraminic acid residues account for ~87% of calcium binding.

    • Conclusions:

    • Rat mastocyte plasma membranes possess high and low affinity calcium binding sites.
    • Phospholipids and neuraminic acid are primary contributors to membrane calcium binding.