Enzymatic conversion of cephamycin C by D-amino acid oxidase from Trigonopsis variabilis

D-Amino acid oxidase (EC 1.4.3.3) systems from Trigonopsis variabilis SANK 59963 were found to catalyze cephamycin C. The reaction products were assigned to 7 beta-(5-carboxy-5-oxovalerylamido)-7 alpha-methoxy-3-carbamoyl-3-cephem-4-carboxylic acid, and 7 beta-(4-carboxy-butyrylamido)-7 alpha-methoxy-3-carbamoyl-3-cephem-4-carboxylic acid, respectively. D-Amino acid oxidase from hog kidney was not able to catalyze cephamycin C.