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Related Experiment Videos

Circulating immune complexes in coccidioidomycosis. Detection and characterization

S Yoshinoya, R A Cox, R M Pope

    The Journal of Clinical Investigation
    |October 1, 1980
    PubMed
    Summary

    Patients with active coccidioidomycosis show elevated levels of circulating immune complexes, which contain fungal antigen and antibody. These immune complexes are not significantly increased in inactive disease, suggesting their role in active fungal infections.

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    Area of Science:

    • Immunology
    • Mycology
    • Infectious Diseases

    Background:

    • Coccidioidomycosis is a fungal infection caused by Coccidioides species.
    • Immune complexes are implicated in various infectious and autoimmune diseases.
    • Detecting immune complexes may aid in diagnosing and understanding coccidioidomycosis pathogenesis.

    Purpose of the Study:

    • To investigate the presence and characteristics of circulating immune complexes in patients with active and inactive coccidioidomycosis.
    • To compare immune complex levels between patients and healthy controls.
    • To develop sensitive assays for detecting fungal antigen-containing immune complexes.

    Main Methods:

    • Sera from patients with active (n=22) and inactive (n=13) coccidioidomycosis and healthy controls (n=54) were analyzed.

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  • Multiple assays were used to detect circulating immune complexes, including Clq-binding and monoclonal rheumatoid factor assays.
  • Sucrose density gradient ultracentrifugation and radioimmunoassay were employed to characterize and quantify immune complexes.

    • Main Results:

    • Elevated circulating immune complexes were detected in 73% of patients with active coccidioidomycosis, versus 13% of controls.
    • Significant increases in immune complexes were observed across all tested assays in active disease (P < 0.05).
    • Immune complexes in active disease were of intermediate size and contained coccidioidin antigen and anticoccidioidin antibody.

    Conclusions:

    • Circulating immune complexes are significantly elevated in active coccidioidomycosis.
    • These immune complexes contain fungal components and may serve as a biomarker for active infection.
    • The developed radioimmunoassay is a sensitive tool for detecting fungal antigen-containing immune complexes in coccidioidomycosis.