Adsorption of antibody and globulin onto glass surfaces

The amount of globulin adsorbed onto surfaces (97 m2) of porous glass (1 g) in phospate-buffered saline (pH 7.2) was estimated to be 83 mg by frontal analysis. In the adsorption chromatography of rabbit antiserum (the immunoglobulin G class) to horse serum albumin on a porous glass column, immunoglobulin G was not eluted with saline but was eluted with 0.2 M glycine (pH 9) with a recovery of 12%. The yield of immunoglobulin M antibody to sheep red blood cells recovered by elution with saline was 12.3%, and the total yield of immunoglobulin M was 15.8%. Thus, antibody and globulin were well adsorbed onto glass surfaces in physiological saline; immunoglobulin G had a stronger affinity to glass surfaces than did immunglobulin M. These facts should be considered when glass containers are used for purified antiserum.