Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Folic acid: crystal structure and implications for enzyme binding

D Mastropaolo, A Camerman, N Camerman

    Science (New York, N.Y.)
    |October 17, 1980
    PubMed
    Summary

    The crystal structure of folic acid dihydrate reveals an extended molecular conformation. This unique structure, determined by X-ray diffraction, offers insights into folate interactions with enzymes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Two rhodamine derivatives: 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis-(ethylamino)-2,7-dimethylxanthylium chloride monohydrate and 3,6-diamino-9-[2-(methoxycarbonyl)-phenyl]xanthylium chloride trihydrate.

    Acta crystallographica. Section C, Crystal structure communications·2001
    Same author

    Crystal structure of the hydrated strontium salt of methotrexate: two independent molecules with different conformations.

    Journal of medicinal chemistry·2001
    Same author

    The antifolate trimetrexate: observation of the enzyme-binding conformation.

    Acta crystallographica. Section C, Crystal structure communications·2000
    Same author

    Ranitidine hydrochloride, a polymorphic crystal form.

    Acta crystallographica. Section C, Crystal structure communications·2000
    Same author

    The antiestrogen [2-(4-benzylphenoxy)ethyl]diethylammonium chloride.

    Acta crystallographica. Section C, Crystal structure communications·2000
    Same author

    Bis(2,2-dimethylaziridinyl)phosphinic amide.

    Acta crystallographica. Section C, Crystal structure communications·1999

    Area of Science:

    • Structural Biology
    • Crystallography
    • Biochemistry

    Background:

    • Folic acid is a vital nutrient essential for DNA synthesis and repair.
    • Understanding the precise molecular structure of folic acid is crucial for comprehending its biological functions and interactions with enzymes.

    Purpose of the Study:

    • To determine the crystal and molecular structure of folic acid dihydrate.
    • To elucidate the conformation of folic acid and its potential interactions within biological systems.

    Main Methods:

    • X-ray diffraction analysis was employed to determine the three-dimensional structure.
    • Detailed analysis of atomic positions and intermolecular interactions was performed.

    Main Results:

    • Folic acid dihydrate adopts an extended molecular conformation.
    • The pteridine ring exists in the keto form, with specific hydrogen bonding interactions involving water molecules.
    • The observed conformation differs significantly from that of methotrexate bound to dihydrofolate reductase, suggesting distinct binding modes.

    Conclusions:

    • The determined structure provides a detailed atomic-level understanding of folic acid.
    • The observed pteridine and phenyl ring stacking suggests potential interactions relevant to folate-enzyme complexes.
    • This structural information is valuable for drug design and understanding folate metabolism.

    Related Experiment Videos