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Related Experiment Videos

Cryopreservation of complement-coated erythrocytes

R W Plunkett, R K Cunningham, S H Lichtenberg

    Transfusion
    |September 1, 1980
    PubMed
    Summary

    Storing complement-coated human red blood cells in liquid nitrogen using a rapid freeze technique is feasible. Frozen cells maintained reactivity comparable to fresh cells when tested with antiglobulin and anticomplement reagents.

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    Immunological investigations·1998

    Area of Science:

    • Immunology
    • Cell Biology
    • Cryopreservation

    Background:

    • Complement components like C3b-C4b and C4b are crucial in immune responses.
    • Preserving complement-coated cells is challenging due to potential degradation during storage.

    Purpose of the Study:

    • To evaluate the feasibility of cryopreserving complement-coated human red blood cells in liquid nitrogen.
    • To assess the reactivity of frozen, stored complement-coated cells.

    Main Methods:

    • Cells were coated with complement components (C3b-C4b and C4b).
    • A low-glycerol, rapid freeze technique was employed for liquid nitrogen storage.
    • Reactivity of frozen cells was compared to freshly coated cells using antiglobulin and anticomplement reagents.

    Main Results:

    • Liquid nitrogen preservation of complement-coated cells was found to be feasible.
    • Frozen, stored cells demonstrated comparable reactivity to freshly prepared cells.
    • The rapid freeze technique maintained the integrity of complement components on red blood cells.

    Conclusions:

    • Cryopreservation of complement-coated red blood cells in liquid nitrogen is a viable method.
    • This technique allows for the long-term storage of complement-sensitized cells without significant loss of reactivity.
    • The findings support the use of frozen, stored complement-coated cells in immunological assays.

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