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Histoautoradiographic studies of 3H-thymidine labelled Erysipelothrix rhusiopathiae introduced into albino mice
Abstract:
S-forms of E. rhusiopathiae were cultivated in a 3H-thimidine-containing medium. A suspension washed three times in succession was intravenously injected into albino mice. At certain intervals liver, spleen and kidney were examinated by routine autoradiogrphy. Intact labelled bacteria were found as early as 5 min after administration in the blood capillaries, around cells of the RES and in some nuclei of hepatocytes. Later they are detected in the cytoplasm of micro- and macrophages, around megacaryocytes and in some of their nuclei. After 6 h the labelled population sharply decreases due to a very active reproduction and mortality of the initial population. The localisation of bacteria in liver nuclei and the spreading of S-forms of E. rhusiopathiae in comparison with the avirulent L-forms therefrom are discussed.
Insights
This study tracked labeled Erysipelothrix rhusiopathiae (S-forms) in mice. Bacteria were found in liver, spleen, and kidney tissues, with significant presence in liver nuclei, indicating complex host-pathogen interactions.
Area of Science:
- Microbiology
- Immunology
- Pathogen Research
Background:
- Erysipelothrix rhusiopathiae causes significant disease in animals.
- Understanding the early distribution and localization of virulent bacterial strains is crucial for disease control.
Purpose of the Study:
- To investigate the in vivo distribution and cellular localization of the S-form of Erysipelothrix rhusiopathiae in mice.
- To compare the spreading patterns of S-forms with avirulent L-forms.
Main Methods:
- Cultivation of S-forms of E. rhusiopathiae in a 3H-thymidine-containing medium.
- Intravenous injection of labeled bacteria into albino mice.
- Autoradiographic examination of liver, spleen, and kidney tissues at various intervals.
Main Results:
- Intact labeled bacteria were detected within 5 minutes in blood capillaries, reticuloendothelial system (RES) cells, and hepatocyte nuclei.
- Later, bacteria were found in the cytoplasm of macrophages and in megakaryocyte nuclei.
- A sharp decrease in the labeled bacterial population was observed after 6 hours, attributed to reproduction and mortality.
Conclusions:
- The S-form of E. rhusiopathiae rapidly disseminates within the host, with a notable localization in liver nuclei.
- The study provides insights into the early pathogenesis and host-cell interactions of E. rhusiopathiae.