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Related Experiment Videos

Embolus radiolabelling in a new canine model

H H Kaufman, J H Anderson, J Woo

    Acta Neurochirurgica
    |January 1, 1980
    PubMed
    Summary

    Radiolabeling emboli with iodine-131 fibrinogen in dogs successfully visualized clot persistence. This technique shows promise for investigating blood clots and preventing strokes.

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    Area of Science:

    • Vascular Surgery
    • Radiopharmacology
    • Experimental Medicine

    Background:

    • Thromboembolism poses a significant risk for ischemic stroke.
    • Developing reliable methods to study emboli in vivo is crucial for understanding clot behavior and developing preventative strategies.

    Purpose of the Study:

    • To investigate the feasibility of radiolabeling emboli using 131I fibrinogen in a canine model.
    • To assess the persistence and detection of artificially created emboli in the internal carotid artery.

    Main Methods:

    • Preparation of canine blood clots by inactivating plasminogen activators and promoting retraction.
    • Induction of internal carotid artery embolization in a canine model.
    • Utilizing 131I fibrinogen for embolus radiolabeling.
    • Employing autoradiography and well counting for isotope detection.

    Main Results:

    • Emboli persisted for 48 hours in 80% of the canine subjects.
    • No major strokes were observed when emboli volumes ranged from 0.25 to 0.30 cm3.
    • Autoradiography and well counting confirmed successful isotope uptake in the emboli.

    Conclusions:

    • Radiolabeling emboli with 131I fibrinogen is a viable method in a canine model.
    • This technique demonstrates potential as a tool for investigating thromboemboli and their behavior in vivo.
    • Further refinement could enhance its utility in preclinical research for stroke prevention.

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