Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urate metabolism in a mongrel dog

P A Simkin

    Advances in Experimental Medicine and Biology
    |January 1, 1980
    PubMed
    Summary

    Urate metabolism in dogs shows linear turnover for urate and allantoin. Renal synthesis and excretion of urate may occur, suggesting multiple urate molecular forms influencing clearance.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessing biomarkers in synovial fluid: consider the kinetics of clearance.

    Osteoarthritis and cartilage·2012
    Same author

    The treatment of hyperuricemia: allopurinol and beyond.

    Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases·2008
    Same author

    A biography of the chondrocyte.

    Annals of the rheumatic diseases·2008
    Same author

    Effect of tumour necrosis factor alpha antagonists on serum transaminases and viraemia in patients with rheumatoid arthritis and chronic hepatitis C infection.

    Annals of the rheumatic diseases·2003
    Same author

    Acetabular osteitis in ankylosing spondylitis: does fibrillin figure in its pathogenesis?

    The Journal of rheumatology·2002
    Same author

    When, why, and how should we quantify the excretion rate of urinary uric acid?

    The Journal of rheumatology·2001

    Area of Science:

    • Biochemistry
    • Renal Physiology
    • Metabolic Studies

    Background:

    • Understanding urate metabolism is crucial for diagnosing and treating conditions like gout.
    • Previous studies have explored urate kinetics, but the specific mechanisms of renal handling require further elucidation.

    Purpose of the Study:

    • To investigate the short-term turnover and renal handling of urate and its metabolite, allantoin, in a canine model.
    • To assess the impact of specific pharmacological agents on urate metabolism.
    • To explore potential differences in the renal clearance of endogenous versus exogenous urate.

    Main Methods:

    • Short-term turnover studies were conducted in a trained mongrel dog.
    • Infusions of Pyrazinamide, Probenecid, and Sodium Salicylate were administered.
    • Urate and allantoin levels, along with isotopic urate clearance, were measured.

    Main Results:

    • Linear turnover curves were observed for both urate and allantoin.
    • Pyrazinamide, Probenecid, and Sodium Salicylate did not significantly affect urate metabolism.
    • The uricolytic index for endogenous urate was lower than for infused isotopic urate, suggesting renal synthesis.
    • Renal clearance of labelled urate exceeded that of endogenous urate, indicating potential existence of multiple urate molecular forms.

    Conclusions:

    • Canine urate metabolism exhibits linear turnover kinetics.
    • Evidence suggests renal synthesis and direct excretion of endogenous urate.
    • Observed differences in renal clearance imply that urate may exist in multiple molecular forms.

    Related Experiment Videos