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Molecular-cellular interactions in the secretory IgA system

S S Crago, S J Prince, R Kulhavy

    Advances in Experimental Medicine and Biology
    |January 1, 1978
    PubMed
    Summary

    Secretory Component (SC) acts as a receptor and transport protein for polymeric immunoglobulins, facilitating their binding to epithelial cells. However, SC is not involved in guiding IgA precursor cells to secretory tissues.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Polymeric immunoglobulins, particularly IgA, play a crucial role in mucosal immunity.
    • The transport of polymeric IgA across epithelial barriers is essential for its function.
    • The role of Secretory Component (SC) in this process has been investigated.

    Purpose of the Study:

    • To investigate the presence and function of Secretory Component (SC) receptors on human lymphoid cells.
    • To determine the role of SC in the binding and transport of polymeric IgA to epithelial cells.
    • To elucidate the involvement of SC in the homing of IgA precursor cells.

    Main Methods:

    • Flow cytometry was used to detect SC receptors on peripheral blood lymphocytes (PBL) and lymphoblastoid cell lines.

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  • Immunofluorescence microscopy was employed to visualize SC binding within cells.
  • Binding assays were performed using human colonic carcinoma HT-29 cells and polymeric IgA.

    • Main Results:

    • SC receptors were absent on the surface of human PBL and lymphoblastoid cell lines, before or after stimulation.
    • SC binding was observed in the cytoplasm of differentiated lymphoid cells, often co-localizing with intracellular IgA.
    • Polymeric IgA binding to the surface of HT-29 epithelial cells was dependent on the presence of SC.

    Conclusions:

    • Secretory Component (SC) functions as a receptor and potential transport protein for polymeric immunoglobulins.
    • SC mediates the binding of polymeric IgA to epithelial cells.
    • SC is not directly involved in the homing of IgA precursor cells to secretory tissues.