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Related Experiment Videos

Lactate dehydrogenase in tears

C MacKay, D H Abramson, R M Ellsworth

    American Journal of Ophthalmology
    |September 1, 1980
    PubMed
    Summary
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    Lactate dehydrogenase (LDH) levels in tears are not a reliable indicator for diagnosing retinoblastoma. Tear LDH levels increase with eyelid rubbing, but this elevation is not consistently linked to the cancer itself.

    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Oncology

    Background:

    • Lactate dehydrogenase (LDH) is an enzyme present in various tissues.
    • Elevated LDH levels can indicate tissue damage or certain diseases.
    • Retinoblastoma is a malignant tumor of the retina.

    Purpose of the Study:

    • To investigate lactate dehydrogenase (LDH) levels in tears.
    • To determine if tear LDH can be a diagnostic marker for retinoblastoma.

    Main Methods:

    • Tear samples were collected from normal subjects and patients with retinoblastoma.
    • LDH levels and isoenzyme profiles were analyzed in tear specimens.
    • Tear collection methods included both non-traumatic and traumatic (eyelid rubbing) approaches.

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    Main Results:

    • Normal subjects typically had undetectable tear LDH without eyelid trauma.
    • Eyelid rubbing in normal subjects significantly increased tear LDH levels (5-10x normal aqueous humor).
    • LDH isoenzyme 5 was consistently higher than LDH isoenzyme 1 in tears, similar to aqueous humor.
    • While some retinoblastoma patients showed elevated tear LDH, the source (epithelial cells vs. tumor) was unclear.

    Conclusions:

    • Tear lactate dehydrogenase (LDH) levels, particularly with current collection methods, are not a reliable diagnostic marker for retinoblastoma.
    • The observed elevations in tear LDH are likely due to epithelial cell release from eyelid manipulation rather than the tumor itself.