[Effect of several endocrine factors on the concentration of specific estrogen-binding protein in rat liver]

Differential determination of specific estrogen-binding protein (SEBP) content in the rat liver was made by ion-exchange sorption on DEAE-cellulose. The concentration of estradiol-binding sites of SEBP in liver cytosol of normal males was 11.1 +/- 1.1 pmole/1 mg protein. SEBP was not detected in the liver of immature males, of mature, mature ovariectomized and mature neonatal androgenized females. Testosterone propionate (TP) could induce SEBP in the liver of ovariectomized females. Maximal induction of SEBP was produced following daily injection of 0.3 mg of TP for 3 days. Single injection of TP had no effect. The induction of SEBP was more sensitive to TP than uterine response but required a more prolonged exposure to the androgen. SEBP was detectable in the liver for at least 43 days after discontinuance of TP injections into ovariectomized females and 56 days after castration of the males.