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Decrease in plasma glucose concentration during storage at -20 degrees C

O Giampietro, R Navalesi, G Buzzigoli

    Clinical Chemistry
    |November 1, 1980
    PubMed
    Summary
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    Plasma glucose levels decrease over time during frozen storage. Longer storage intervals at -20°C significantly correlate with greater percentage decreases in glucose concentration.

    Area of Science:

    • Clinical Chemistry
    • Biomedical Diagnostics
    • Endocrinology

    Background:

    • Accurate glucose measurement is crucial for diagnosing and managing diabetes.
    • Plasma sample stability during storage can impact diagnostic accuracy.
    • Understanding glucose degradation kinetics is essential for reliable clinical testing.

    Purpose of the Study:

    • To investigate the effect of frozen storage duration on plasma glucose concentrations.
    • To determine the relationship between storage interval and glucose degradation at -20°C.
    • To assess the impact of storage on samples from individuals with varying carbohydrate tolerance.

    Main Methods:

    • Plasma samples from oral glucose tolerance tests and diabetic controls were analyzed.
    • Glucose concentrations were measured using the Beckman Glucose Analyzer.

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  • Samples were stored at -20°C for varying intervals before re-analysis.

    • Main Results:

    • A statistically significant positive correlation (p < 0.001) was observed between storage interval and the percentage decrease in glucose concentration.
    • The longer the plasma samples were stored, the more pronounced the reduction in measured glucose levels.
    • This trend was evident across samples from normal subjects, impaired carbohydrate tolerance patients, and insulin-dependent diabetics.

    Conclusions:

    • Frozen storage of plasma at -20°C leads to a time-dependent decrease in glucose concentration.
    • Extended storage intervals compromise the accuracy of plasma glucose measurements.
    • Clinical laboratories should consider storage duration when interpreting glucose results from frozen samples.