A simplified method for studies of haemoglobin biosynthesis

A simplified method for studies of haemoglobin chain biosynthesis using 35S-methionine as the labeled amino acid and Cellogel electrophoresis for globin chain separation is described. This technique simplifies the procedure globin preparation, improves the electrophoretic separation of globin chains and may be applied to samples with normal reticulocyte counts. As many as 20 samples can be prepared simultaneously and 10 samples can be subjected to electrophoretic separation at one time. The method was fund to yield the expected synthetic ratios and to be highly reproducible when applied to normal and abnormal samples from peripheral blood or bone marrow.