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Isolation of subclasses of human IgG with affinity chromatography
Journal of Immunological Methods
|January 1, 1980
Abstract:
A method is described for the isolation of subclasses of human IgG. Two myeloma proteins, after isolation by DEAE-Sephadex chromatography, were further purified on subclass-specific anti-IgG1 and 2 Sepharose 4B columns. Contaminating IgG1 and 2 were removed from the isolated myeloma proteins.