Role of attachment factors and attractants in fibroblast chemotaxis

Fibroblasts require both a collagen substratum and fibronectin for adherence and subsequent migration in the Boyden chamber. Both fibronectin and lymphokines are good chemoattractants, whereas the collagens are weaker. Fibroblasts appear to require cytoskeletal organization and methylation reactions for chemotaxis, properties shown previously for macrophages and neutrophils. Fibroblasts, however, migrate more slowly than phagocytic cells and appear to require protein synthesis.