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Related Experiment Videos

Histamine formation by mouse preimplantation embryos

S K Dey, D C Johnson

    Journal of Reproduction and Fertility
    |November 1, 1980
    PubMed
    Summary

    Mouse embryos produce histamine, a crucial molecule for their development. Inhibiting histamine production significantly hinders embryo growth, indicating its necessity for successful pregnancy.

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    Area of Science:

    • Reproductive biology
    • Developmental biology
    • Biochemistry

    Background:

    • Histamine plays various roles in mammalian physiology.
    • The specific role of histamine in early embryonic development is not fully understood.

    Purpose of the Study:

    • To investigate whether mouse embryos produce histamine.
    • To determine the role of embryo-produced histamine in early development.

    Main Methods:

    • Measuring histamine production in Day 4 mouse embryos using radiolabeled L-histidine.
    • Assessing blastocyst development in vitro with and without histidine decarboxylase inhibitors.

    Main Results:

    • Day 4 mouse embryos produced histamine at a rate of 1.5 pmol/embryo/hour.
    • Inhibiting histidine decarboxylase with DL-alpha-methylhistidine significantly reduced blastocyst formation.
    • L-histidine supplementation did not affect development, but it reversed the inhibitory effect of DL-alpha-methylhistidine.

    Conclusions:

    • Mouse embryos possess the capacity to synthesize histamine.
    • Embryonic histamine production is essential for normal blastocyst development in vitro.
    • Histamine may be a critical factor in successful early pregnancy.

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