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A streptomycin dependent live Pasteurella multocida vaccine for the prevention of rabbit pasteurellosis
Abstract:
A culture of Pasteurella multocida was isolated from a rabbit exhibiting clinical signs of respiratory pasteurellosis. Organisms from this culture were incubated in the presence of the mutagen n-methyl-n'-nitro-n-nitrosoguandine in order to obtain a streptomycin dependent mutant. A live vaccine was prepared from this mutant, and healthy pasteurella-free rabbits were vaccinated either by intranasal or subcutaneous administration. Either method provided complete protection against homologous, wild-type challenge as evidenced by absence of clinical signs or gross pathology and failure to isolate the organism from mucous secretions of tissues from vaccinated individuals.
Insights
A novel streptomycin-dependent Pasteurella multocida mutant was developed as a live vaccine. Vaccination via intranasal or subcutaneous routes completely protected rabbits against respiratory pasteurellosis.
Area of Science:
- Veterinary Medicine
- Microbiology
- Immunology
Background:
- Pasteurella multocida causes respiratory pasteurellosis in rabbits.
- Developing effective vaccines is crucial for controlling rabbit respiratory diseases.
Purpose of the Study:
- To develop and evaluate a live streptomycin-dependent Pasteurella multocida vaccine for rabbits.
- To assess the efficacy of intranasal and subcutaneous vaccination routes.
Main Methods:
- Isolation of Pasteurella multocida from infected rabbits.
- Induction of streptomycin dependence using N-methyl-N'-nitro-N-nitrosoguanidine.
- Vaccination of healthy rabbits via intranasal or subcutaneous routes.
- Challenge with wild-type Pasteurella multocida.
Main Results:
- A streptomycin-dependent mutant of Pasteurella multocida was successfully generated.
- Both intranasal and subcutaneous vaccination provided complete protection against homologous challenge.
- Vaccinated rabbits showed no clinical signs, gross pathology, or organism recovery.
Conclusions:
- A live, streptomycin-dependent Pasteurella multocida vaccine is effective in preventing respiratory pasteurellosis in rabbits.
- Intranasal and subcutaneous administration routes are both viable for vaccination.
- This vaccine represents a promising tool for controlling pasteurellosis in rabbit populations.