Localization of[3H]-2-deoxyglucose in single molluscan neurones

The glucose analogue 2-deoxyglucose (2-DG) can be used quantitatively to measure metabolic activity and is widely used qualitatively for mapping functional activity in the brain. The resolution (meaning the full width at half maximum of the grain density distribution around a line source) of the technique using [14C]-2-DG and X-ray film is limited to about 100 micrometers. Attempts have been made to improve the resolution using [3H]-2-DG (ref. 6) and cellular resolution has been achieved in the goldfish retina and with cultured mouse neurones. An anatomical technique for mapping the metabolic activity of individual neurones would be useful for studying invertebrate central nervous systems, which are relatively simple and stereotyped compared to vertebrate brains. The [3H]-2-DG technique was applied to an invertebrate in a study of the Drosophila visual system, though without cellular resolution. We present here modifications of the [3H]-2-DG technique to demonstrate localization of 2-DG in single neurones of Limax maximus, a gastropod mollusc, with a resolution of less than 1 micrometer.