Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Activation of complement by Naegleria

B Rowan-Kelly, A Ferrante, Y H Thong

    Transactions of the Royal Society of Tropical Medicine and Hygiene
    |January 1, 1980
    PubMed
    Summary

    Naegleria does not directly attract human neutrophils. However, Naegleria activates human serum complement, generating a strong chemotactic signal, particularly via the alternative pathway.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Longitudinal Flow Decorrelations in Xe+Xe Collisions at sqrt[s_{NN}]=5.44  TeV with the ATLAS Detector.

    Physical review letters·2021
    Same author

    Search for Heavy Resonances Decaying into a Photon and a Hadronically Decaying Higgs Boson in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

    Physical review letters·2021
    Same author

    Search for Higgs Boson Decays into a Z Boson and a Light Hadronically Decaying Resonance Using 13 TeV pp Collision Data from the ATLAS Detector.

    Physical review letters·2020
    Same author

    Dijet Resonance Search with Weak Supervision Using sqrt[s]=13  TeV pp Collisions in the ATLAS Detector.

    Physical review letters·2020
    Same author

    Population Data Centre Profiles: Centre for Data Linkage.

    International journal of population data science·2020
    Same author

    CP Properties of Higgs Boson Interactions with Top Quarks in the tt[over ¯]H and tH Processes Using H→γγ with the ATLAS Detector.

    Physical review letters·2020

    Area of Science:

    • Immunology
    • Microbiology
    • Cell Biology

    Background:

    • Naegleria are free-living amoebae.
    • Neutrophils are key immune cells involved in pathogen defense.

    Purpose of the Study:

    • To investigate the chemotactic interaction between Naegleria and human neutrophils.
    • To determine the role of human serum and complement in this interaction.

    Main Methods:

    • Chemotaxis assays were performed using Naegleria and human neutrophils.
    • Human serum was heat-inactivated to assess complement dependence.
    • C2-deficient serum was used to investigate the alternative complement pathway.

    Main Results:

    • Naegleria and its culture supernatant showed no direct chemotaxis for neutrophils.
    • Interaction of Naegleria with normal human serum generated a potent chemotactic stimulus.
    • Heat inactivation of serum abolished the chemotactic activity, indicating complement involvement.
    • Naegleria activated the alternative complement pathway, as shown by experiments with C2-deficient serum.

    Conclusions:

    • Naegleria does not directly attract neutrophils.
    • Naegleria interacts with human serum to generate a neutrophil chemoattractant.
    • Complement activation, specifically via the alternative pathway, is crucial for this chemoattractant generation.

    Related Experiment Videos