[Virus-specific protein synthesis in cells infected with Japanese encephalitis virus variants]

In BHK-21 and chick embryo cells infected with Japanese encephalitis virus clones, 6-7 virus-specific proteins were identified having molecular weights of 96-98, 71-74, 52-56, 22, 20, 12.5, and 10 kilodaltons (kd). After additional treatment of the infected cells with cycloheximide (300 micrograms/ml for 30 min) combined with a long-term treatment with actinomycin D (1 microgram/ml) three virus-specific proteins with the highest molecular weights were found in the cell lysate. Proteins with moleculr weights 52-56, 22, and 20 kd are glycoproteins; 14C-glucosamine in trace amounts is also incorporated into proteins with molecular weights 96-98 and 71-74 kd. Differences in the molecular weight of the intracellular protein B3 in the clones under study were found.