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Testosterone metabolism in human bone

H U Schweikert, W Rulf, N Niederle

    Acta Endocrinologica
    |October 1, 1980
    PubMed
    Summary
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    Dihydrotestosterone is the active androgen in human bone, not testosterone. Its formation rate in bone tissue is similar in normal and osteoporotic bone, indicating no significant difference in androgen metabolism.

    Area of Science:

    • Endocrinology
    • Bone Biology
    • Biochemistry

    Background:

    • Testosterone is a primary androgen, but its active form in target tissues is often dihydrotestosterone.
    • The role of dihydrotestosterone in human bone metabolism, particularly in osteoporosis, requires further elucidation.

    Purpose of the Study:

    • To investigate the conversion of testosterone to dihydrotestosterone in human bone tissue.
    • To compare the rate of dihydrotestosterone formation in normal versus osteoporotic bone.
    • To determine the active intracellular androgen mediating effects in human bone.

    Main Methods:

    • Human bone samples (spongiosa) were obtained from patients undergoing orthopedic surgery.
    • Radioisotope ([1,2,6,7-3H]testosterone) was used to measure the conversion rate to [3H]dihydrotestosterone.

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  • Enzyme kinetics, including substrate concentration for half-maximum rate, were analyzed.

    • Main Results:

    • Dihydrotestosterone formation was detected in all examined bone samples.
    • The half-maximum rate of dihydrotestosterone formation occurred at 0.3 microM substrate concentration, similar to other androgen target organs.
    • No significant difference was observed in the rate of dihydrotestosterone formation between normal and osteoporotic bone.
    • Testosterone to androstenedione conversion rates were also similar in both bone types.

    Conclusions:

    • Dihydrotestosterone is likely the active intracellular androgen in human bone.
    • The 5 alpha-reductase enzyme, responsible for converting testosterone to dihydrotestosterone, appears functional in human bone.
    • Osteoporosis does not significantly alter the rate of dihydrotestosterone formation in bone compared to normal bone.