Related Experiment Video
Updated: Aug 9, 2026

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
Published on: October 29, 2012
Release of acetylcholine from the brain in vivo: some comments on estimation methods and their application
Abstract:
Techniques (push-pull cannula, cup, brain ventricles perfusion) allowing estimation of the amount of the ACh released in vivo from the brain are described. The main attention is paid to biochemical, physiological and morphological factors influencing the amount of ACh released and available for estimation. Conditions of experiments for each of these techniques are described in details. The amount of ACh released in different physiological states of several animal species is compared. Typical applications of all three methods are given.
Related Concept Videos
Cholinergic Neurons: Neurotransmission
Cholinergic Receptors: Muscarinic
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...
Direct-Acting Cholinergic Agonists: Pharmacokinetics
Cholinesterases: Distribution and Function

