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Related Experiment Videos

Site-specific recombination in bacteriophage lambda: structural analyses of reactive DNA sequences

A Landy, P L Hsu, W Ross

    The American Journal of Tropical Medicine and Hygiene
    |September 1, 1980
    PubMed
    Summary

    Bacteriophage lambda recombination uses unequal DNA partners. The phage att site, a donor, is larger than the bacterial att site recipient, with distinct Int binding sites crucial for function.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Site-specific recombination is essential for bacteriophage lambda integration into host genomes.
    • The bacteriophage lambda att site mediates this integration process, interacting with bacterial DNA.
    • Understanding the att site's structure and function is key to deciphering viral integration mechanisms.

    Purpose of the Study:

    • To characterize the structural and functional properties of the bacteriophage lambda att site.
    • To investigate the nature of the DNA partners involved in integrative recombination.
    • To propose a model for integrative recombination based on partner asymmetry.

    Main Methods:

    • Analysis of bacteriophage lambda att site structure, including Int binding sites.

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  • Assessment of Int binding site characteristics, such as size and heparin binding.
  • Comparison of phage att site requirements with potential bacterial att site partners.

    • Main Results:

    • The bacteriophage lambda att site is approximately 240 base pairs with four distinct Int binding sites of two size classes (30-35 bp and 15 bp).
    • Three of the four Int binding sites are essential for att site function.
    • The minimal bacterial att site partner sequence may be as small as the 15 bp common core.

    Conclusions:

    • Integrative recombination in bacteriophage lambda involves two partners of unequal size and complexity.
    • The phage att site acts as a larger, more complex 'donor' site.
    • The bacterial att site functions as a smaller 'recipient' site, highlighting an asymmetric recombination model.