Growth of hebdomadis group of leptospires in solid medium

Leptospira hardjo, szwajizak, and sejroe produced colonial growth in bovine albumin polysorbate 80 (BAP80) solid medium which had been supplemented either with sodium pyruvate (100 micrograms/ml) or with 5% rabbit serum. Leptospira hardjo produced two morphologic types of colonies in both supplemented media. Growth did not occur with any of the three serovars in the unsupplemented BAP80 medium. These results indicate that addition of either rabbit serum or sodium pyruvate would aid in promoting growth of some fastidious leptospires.