[Growth in vitro of human bone marrow after freezing in liquid nitrogen: analysis of a case]

Human marrow is frozen according to the Schafer's technique; CFU-C proliferation and differentiation is then evaluated in relation to the time of storage in liquid nitrogen and to the time of in vitro culture in agar system according to the Pike and Robinson's technique. The highest plating efficiency is observed at the twelfth day of culture while in fresh cell suspension the growth peaks at the eighth day.