A comparison of histone variants in different rat tissues

The histone patterns of rat liver, spleen, kidney and brain were compared by means of a two-dimensional electrophoresis in the presence of Triton X-100. In addition, a two-step procedure was introduced for quantitative estimation of different histone variants. Tissue differences wee found in the relative content of the different variants of H2a, H3 and the subfraction H1O. It was also found that the high salt induced H2a specific proteolytic activity was very high in spleen, very low in liver and kidney and absent in brain.