Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aromatase activity in the developing rabbit brain

F W George, W T Tobleman, L Milewich

    Endocrinology
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of castration and androgen treatment on androgen-receptor levels in rat skeletal muscles.

    Journal of applied physiology (Bethesda, Md. : 1985)·1999
    Same author

    Virilization of the male pouch young of the tammar wallaby does not appear to be mediated by plasma testosterone or dihydrotestosterone.

    Biology of reproduction·1999
    Same author

    Identification of genes expressed in the rat prostate that are modulated differently by castration and Finasteride treatment.

    The Journal of endocrinology·1998
    Same author

    Androgen metabolism in the prostate of the finasteride-treated, adult rat: a possible explanation for the differential action of testosterone and 5 alpha-dihydrotestosterone during development of the male urogenital tract.

    Endocrinology·1997
    Same author

    Regulation of immunoreactive androgen receptor in the adrenal gland of the adult rat.

    Endocrinology·1996
    Same author

    Women with steroid 5 alpha-reductase 2 deficiency have normal concentrations of plasma 5 alpha-dihydroprogesterone during the luteal phase.

    The Journal of clinical endocrinology and metabolism·1995

    Rabbit embryos show significant aromatase activity in the forebrain, particularly the diencephalon, suggesting it is a key source of estrogens during development. This brain region

    Area of Science:

    • Reproductive Biology
    • Developmental Neuroscience
    • Endocrinology

    Background:

    • Estrogens play crucial roles in embryonic development.
    • The sources and regulation of embryonic estrogen production are not fully understood.
    • Aromatase is the key enzyme responsible for converting androgens to estrogens.

    Purpose of the Study:

    • To investigate aromatase activity in rabbit placenta and central nervous system during embryonic development.
    • To determine the specific brain regions and developmental stages with significant estrogen synthesis.
    • To compare the estrogen-synthesizing capacity of the fetal brain with other organs.

    Main Methods:

    • Measurement of 17beta-[3H]estradiol formation from [1,2,6,7-3H]testosterone in tissue homogenates.

    Related Experiment Videos

  • Assay of aromatase activity in placental and various central nervous system tissues from rabbit embryos (13-28 days gestation).
  • Quantification of enzyme activity per milligram of protein and projected to whole organ capacity.

    • Main Results:

    • Significant aromatase activity was localized to the fetal forebrain, peaking in the diencephalon between days 19-25 of gestation.
    • Diencephalon aromatase activity per milligram of protein was second only to the fetal ovary.
    • Whole organ aromatization capacity of the diencephalon exceeded that of the fetal ovary approximately ninefold.
    • Placental aromatase activity was high early in gestation (day 13) but decreased significantly by day 19.

    Conclusions:

    • The fetal forebrain, particularly the diencephalon, is a significant site of estrogen synthesis in rabbit embryos.
    • The diencephalon's capacity for aromatization suggests a crucial role in supplying estrogens during embryogenesis.
    • These findings highlight the potential importance of brain-derived estrogens in regulating embryonic development.