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Updated: Aug 25, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Modified hybridoma methodology: antigen-directed chemically mediated cell fusion

D M Kranz, P A Billing, J N Herron

    Immunological Communications
    |January 1, 1980
    PubMed

    The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multi-cellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.

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