A multi-channel system for the measurement of spermatozoan collective motility

A new multi-channel optical system for objective evaluation of sperm motility is described. The principle of the technique is based on the measurement of changes in the intensity of light scattered from semen samples thermoregulated at 38 degrees C. These changes were recorded continuously as analog waves (Reflectospermiogram--RSG). Changes in the intensity of sperm motility as a function of time from ejaculation were simultaneously measured in 8 equal semen samples from single ejaculate. By analyzing the variations in results, the sensitivity of the technique was defined. In order to demonstrate the use of the technique, the effect of various concentrations of ethanol on sperm motility has been described. The exposure of sperm cells to 366 nm light used in the system did not alter their ultrastructure, as was observed by electron microscopy. This objective, relatively easy to perform and highly informative technique can be of great value in research studies.