The effect of pH on ram sperm collective motility driven by mitochondrial respiration

Ram sperm cells treated with 0.02 M 2-deoxy-D-glucose exhibit collective motility which is driven only by mitochondrial respiration. This motility was measured, by means of multichannel optical objective technique, under various [H+] concentrations (pH range 5.6-7.2) buffered by 0.125 M phosphate buffers. Semen was divided into 8 aliquots and motility was monitored simultaneously for intensity and duration within 10 min of ejaculation until its complete cessation. The optimal pH range found for the two above-mentioned parameters was in the range of 6.0-6.5. A certain characteristic of the pattern of sperm motility intensity (an oscillations phenomenon) was similarly related to pH. It is suggested that the mechanisms underlying the relationship between pH and motility are rather metabolic. Concerning the results, some practical implications are introduced.