L-proline as a glutamate antagonist at a crustacean neuromuscular junction

The fast as well as the slow contractions of the adductor muscle in the claw of Procambarus clarkii are inhibited by L-proline. This inhibition is dose dependent and decreases with increasing frequency of stimulation of the "slow" fiber. Contractions caused by perfusing the adductor muscle with L-glutamate solutions are also inhibited by L-proline. The inhibiting potency of L-proline is small; the effective concentration of this amino acid is 50--100 times that of the L-glutamate applied. It was postulated that the inhibitory effect of L-proline is based on competition for excitatory receptor sites of L-glutamate, which causes depolarization and contraction, and L-proline, which lacks these actions. Theoretical considerations suggested a linear relationship between the stimulating L-glutamate and the just-inhibiting L-proline concentrations. Experimental evidence supported this model.