Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Voltage-sensitive outward currents in cat motoneurones

E F Barrett, J N Barrett, W E Crill

    The Journal of Physiology
    |July 1, 1980
    PubMed
    Summary

    Cat motoneurones exhibit two distinct voltage-gated potassium conductances activated by depolarization, influencing action potential dynamics. A third conductance, activated by hyperpolarization, contributes to anomalous rectification in these neurons.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Overexpression of Bcl-xl protects septal neurons from prolonged hypoglycemia and from acute ischemia-like stress.

    Neuroscience·2005
    Same author

    Initiation and propagation of regenerative Ca(2+)-dependent potentials in dendrites of layer 5 pyramidal neurons.

    Journal of neurophysiology·2001
    Same author

    Dendritic calcium spikes in layer 5 pyramidal neurons amplify and limit transmission of ligand-gated dendritic current to soma.

    Journal of neurophysiology·2001
    Same author

    Contrasting contributions of endoplasmic reticulum and mitochondria to Ca(2)+ handling in neurons.

    The Journal of general physiology·2001
    Same author

    Bone morphogenetic proteins (BMP6 and BMP7) enhance the protective effect of neurotrophins on cultured septal cholinergic neurons during hypoglycemia.

    Journal of neurochemistry·2001
    Same author

    Stimulation-evoked increases in cytosolic [Ca(2+)] in mouse motor nerve terminals are limited by mitochondrial uptake and are temperature-dependent.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·2000

    Area of Science:

    • Neuroscience
    • Electrophysiology
    • Computational Biology

    Background:

    • Motoneurones are crucial for motor control, and understanding their electrical properties is essential.
    • Voltage-gated ion channels, particularly potassium channels, play a significant role in shaping neuronal excitability and action potentials.

    Purpose of the Study:

    • To characterize the voltage-dependent potassium conductances in cat motoneurones using in vivo voltage clamp techniques.
    • To elucidate the kinetic properties and functional roles of these conductances in action potential generation and propagation.

    Main Methods:

    • In vivo voltage clamp of cat motoneurone soma using intracellular electrodes.
    • Analysis of activation and decay kinetics of potassium conductances.
    • Reconstruction of action potential features using simulations based on voltage clamp data.

    Main Results:

    • Identified two distinct depolarization-activated potassium conductances with different activation/decay rates and a common reversal potential.
    • Characterized a hyperpolarization-activated conductance contributing to anomalous rectification.
    • Simulations accurately reconstructed motoneurone action potentials, highlighting the role of fast K+ conductance and dendritic capacitive currents.

    Conclusions:

    • Cat motoneurones possess multiple, distinct voltage-gated potassium conductances that differentially regulate neuronal excitability.
    • These conductances are critical for shaping the action potential waveform and contributing to phenomena like anomalous rectification.
    • Computational models based on experimental data provide valuable insights into motoneurone electrophysiology.

    Related Experiment Videos