Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Feline calicivirus induced polypeptides

O O Komolafe, O Jarrett, J C Neil

    Microbios
    |January 1, 1980
    PubMed
    Summary

    Researchers identified a novel 80,000-dalton protein in feline calicivirus-infected cells. This non-structural protein lacks a precursor-product relationship with known viral capsid proteins, suggesting a new role in viral replication.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Runx1 Orchestrates Sphingolipid Metabolism and Glucocorticoid Resistance in Lymphomagenesis.

    Journal of cellular biochemistry·2016
    Same author

    Buruli ulcer in Malawi - a first report.

    Malawi medical journal : the journal of Medical Association of Malawi·2016
    Same author

    Antibiotic resistance in bacteria - an emerging public health problem.

    Malawi medical journal : the journal of Medical Association of Malawi·2016
    Same author

    Evaluation of syndromic management of sexually transmitted infections in Blantyre, Malawi.

    Malawi medical journal : the journal of Medical Association of Malawi·2016
    Same author

    Angiomyolipoma presenting as renal abscesses: a consideration for further evaluation of renal abscess.

    The Nigerian postgraduate medical journal·2013
    Same author

    Awareness and knowledge of glaucoma among workers in a Nigerian tertiary health care institution.

    Middle East African journal of ophthalmology·2013

    Area of Science:

    • Virology
    • Molecular Biology
    • Protein Chemistry

    Background:

    • Feline calicivirus (FCV) is a significant pathogen in cats.
    • Understanding FCV protein synthesis is crucial for developing antiviral strategies.
    • Previous studies focused on capsid protein synthesis and their precursors.

    Purpose of the Study:

    • To identify and characterize non-structural proteins synthesized during FCV infection.
    • To investigate the relationship between novel polypeptides and FCV capsid proteins.
    • To contribute to the understanding of FCV replication mechanisms.

    Main Methods:

    • Analysis of feline calicivirus-infected cell extracts.
    • Protein separation using molecular weight determination (e.g., SDS-PAGE).
    • Investigation of precursor-product relationships between viral proteins.

    Main Results:

    • A submolar amount of an 80,000-dalton polypeptide was detected.
    • This 80-kDa polypeptide showed no precursor-product relationship with FCV capsid proteins (68-kDa and 14-kDa).
    • Two additional highly labeled non-structural polypeptides (80-kDa and 40-kDa) were identified but remain unidentified.

    Conclusions:

    • The 80-kDa polypeptide is a novel non-structural protein in FCV-infected cells.
    • This protein is distinct from FCV capsid proteins and their precursors.
    • Further research is needed to identify the function of these newly discovered non-structural proteins.

    Related Experiment Videos