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A nitrogen pressure-shearing apparatus for preparing distinct populations of chromatin fractions
Preparative Biochemistry
|January 1, 1980
Abstract:
A method for preparing reproducible chromatin fractions is described. Fragmentation is accomplished by expelling whole chromatin from an enclosed stainless-steel chamber through a restricted orifice utilizing low nitrogen pressure. Sheared chromatin is fractionated into two distinct heavy and light fractions by centrifugation on 5-20% linear sucrose gradient.