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Related Experiment Videos

Falsely negative urinary leucocyte counts due to delayed examination

H Kierkegaard, U Feldt-Rasmussen, M Hørder

    Scandinavian Journal of Clinical and Laboratory Investigation
    |May 1, 1980
    PubMed
    Summary

    Urine sample analysis for leukocytes requires prompt testing. Delaying microscopic examination of urinary leukocytes by 3 hours led to 35% of positive results becoming negative, highlighting the need for rapid testing.

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    Area of Science:

    • Clinical chemistry
    • Urology
    • Medical diagnostics

    Background:

    • Accurate detection of urinary leukocytes is crucial for diagnosing urinary tract infections.
    • The stability of leukocytes in urine samples can be affected by time and storage conditions.

    Purpose of the Study:

    • To evaluate the impact of delayed microscopic examination on the accuracy of urinary leukocyte counts.
    • To determine the optimal time frame for analyzing urine samples for leukocytes after collection.

    Main Methods:

    • Microscopic examination of urinary leukocytes was conducted immediately after micturition (in-ward) and approximately 3 hours later (in-laboratory).
    • Forty-eight urine specimens were analyzed, comparing results from immediate and delayed testing.

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    Main Results:

    • A significant discrepancy was observed between in-ward and in-laboratory counts.
    • 35% of specimens initially positive for leukocytes when tested immediately became negative upon delayed laboratory examination.
    • This indicates a substantial loss of detectable leukocytes over a 3-hour period.

    Conclusions:

    • Microscopic counting of urinary leukocytes should be performed as soon as possible after micturition to ensure accurate results.
    • Testing within 1 hour of sample collection is recommended to minimize leukocyte degradation and potential false-negative findings.