Individual difference in non-hemoglobin proteins of red cell lysate

Non-hemoglobin proteins (NHP) were effectively isolated from red cell lysate by CM-Sephadex chromatography using 0.01 M phosphate buffer pH 6.3. NHP from 180 subjects was analyzed with polyacrylamide gel disc electrophoresis. The individual difference was observed at the intermediately migrating zone after protein staining. Group I had two of the three bands migrating quickly, group II all the three bands, and group III two of the three bands migrating slowly. Incidences of groups I, II and III were 67, 20 and 13 percent, respectively.