Methods for 3H-2-D-deoxyglucose autoradiography on film and fine-grain emulsions

Deoxyglucose labelled with 14C is utilized for the determination of regional glucose metabolism in the brain. In this paper, procedures are described in which 3H replaces the 14C marker for film autoradiography. A significant improvement of resolution is obtained as a result of the use of tritium and of various precautions to avoid diffusion of the soluble deoxyglucose. In addition, a method using a fine-grain emulsion (NTB-2) is presented. In this case, definition of labelled sites at a cytological level is obtained. Precise quantification is also possible through grain counting.