Demonstration of thymus-leukemia (TL) antigens on mitochondria of lymphoid cells by immunoelectron microscopy
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The presence of thymus-leukemia (TL) antigens on the surfaces of mitochondria isolated from TL+ cells, e.g., RADA 1 leukemia cells, and TL+ normal thymocytes, has been directly demonstrated by immunoelectron microscopy. Reagents used were TL alloantiserum and a hemocyanin conjugate of rabbit antibody to mouse IgG. No hemocyanin labeling was observed on mitochondria obtained from TL- cells, e.g., thymocytes of TL- strains, splenocytes of either TL+ or TL- strains, and TL- leukemia cells. The concurrence of TL antigens on the plasma and mitochondrial membranes was shown by the ability of intact cells to absorb all reactivity toward mitochondria.
The presence of thymus-leukemia (TL) antigens on the surfaces of mitochondria isolated from TL+ cells, e.g., RADA 1 leukemia cells, and TL+ normal thymocytes, has been directly demonstrated by immunoelectron microscopy. Reagents used were TL alloantiserum and a hemocyanin conjugate of rabbit antibody to mouse IgG. No hemocyanin labeling was observed on mitochondria obtained from TL- cells, e.g., thymocytes of TL- strains, splenocytes of either TL+ or TL- strains, and TL- leukemia cells. The concurrence of TL antigens on the plasma and mitochondrial membranes was shown by the ability of intact cells to absorb all reactivity toward mitochondria.