Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Development and experimental study of a dry selective medium for staphylococci]

B M Raskin, S M Shtanchaeva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |September 1, 1980
    PubMed
    Summary

    A novel dried selective medium using expired hydrolysin and aminopeptide effectively cultivates staphylococci. High salt concentration inhibits other microbes, enabling pigment and lecithinase activity determination.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [The development of Russian nutrient media for isolating the recombinant surface antigen of the hepatitis B virus].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·1994
    Same author

    [Design of a nutrient medium for bacteria of the genus Haemophilus--producers of restriction endonucleases].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·1989
    Same author

    [Possibility of the development of low-waste production of sera and the rational use of recycled materials].

    Gigiena i sanitariia·1988
    Same author

    [A new dried nutrient medium for the isolation of Corynebacterium diphtheriae (its development and experimental study)].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·1988
    Same author

    [The problem of nutrient media today].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·1988
    Same author

    [Effect of the composition of the nutrient media and culture conditions on the growth of strains of Campylobacter jejuni and C. coli].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·1988

    Area of Science:

    • Microbiology
    • Food Safety

    Background:

    • Staphylococci are significant in food safety and clinical settings.
    • Selective media are crucial for isolating staphylococci from mixed microbial populations.

    Purpose of the Study:

    • To develop a new dried selective medium for staphylococci cultivation.
    • To assess the medium's efficacy in inhibiting extraneous microflora.
    • To evaluate its utility for determining staphylococcal pigment formation and lecithinase activity.

    Main Methods:

    • Formulation of a dried selective medium using expired hydrolysin and aminopeptide.
    • Incorporation of high-concentration sodium chloride to inhibit competing microorganisms.
    • Testing the medium's performance as milk-yolk-salt agar for specific staphylococcal characteristic determination.

    Main Results:

    • The developed dried medium successfully supported staphylococci growth.
    • High salt concentration effectively suppressed the growth of extraneous microflora.
    • The milk-yolk-salt agar formulation allowed for the determination of pigment formation and lecithinase activity.

    Conclusions:

    • A novel, effective dried selective medium for staphylococci has been developed.
    • The medium's composition, including high salt content, ensures selective isolation.
    • The milk-yolk-salt agar variant is suitable for characterizing staphylococcal virulence factors.

    Related Experiment Videos