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Related Experiment Videos

Applanation tonometry with and without fluorescein

D L Roper

    American Journal of Ophthalmology
    |November 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Fluorescein staining is essential for accurate applanation tonometry. Omitting fluorescein during intraocular pressure measurement leads to a significant underestimation of the actual eye pressure.

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    Area of Science:

    • Ophthalmology
    • Optometry

    Background:

    • Applanation tonometry is a standard method for measuring intraocular pressure (IOP).
    • Some practitioners question the necessity of fluorescein staining for this procedure.

    Purpose of the Study:

    • To determine the impact of fluorescein staining on the accuracy of applanation tonometry.
    • To evaluate the difference in IOP measurements with and without fluorescein.

    Main Methods:

    • Intraocular pressure was measured using applanation tonometry in eyes with and without fluorescein staining.
    • Measurements were taken sequentially on the same eyes to compare results.

    Main Results:

    • Eyes measured without fluorescein showed an average pressure of 11.72 +/- 3.20 mm Hg.

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  • Eyes immediately remeasured with fluorescein showed a significantly higher average pressure of 17.34 +/- 4.07 mm Hg.
  • Failure to use fluorescein resulted in an average underestimation of 5.62 mm Hg.

    • Conclusions:

    • Fluorescein staining is critical for accurate visualization of the tear meniscus and applanated area.
    • Omitting fluorescein during applanation tonometry leads to clinically significant underestimation of IOP.