Adenosinetriphosphate (ATP) in human spermatozoa. II. Concentrations in fertile men

The Bucher's method was applied to establish normal values of ATP in the spermatozoa of fertile men. It was shown that the normal value is 6.5 +/- 0.34 S.E. microgram per 10(8) spermatozoa. Pools of spermatozoa showed that incubation in seminal plasma at room temperature produced a progressive loss of ATP in the gametes.